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Serologic testing for verifying immunization or past infection

Serologic testing for verifying immunization or past infection
  Test Protective level
Children ≥5 months of age
Diphtheria antibody (IgG) ELISA >0.10 IU/mL
Tetanus antibody (IgG) ELISA >0.10 IU/mL
Hepatitis B surface antibody (IgG) ELISA ≥10 mIU/mL
Haemophilus influenzae type b polyribosylribitol phosphate antibody (IgG)* ELISA ≥1.0 IU/mL
Children ≥12 months of age
Rubeola (measles) antibody (IgG) IFA or ELISA
Mumps antibody (IgG) IFA or ELISA
Rubella antibody (IgG) ELISA
Varicella antibody (IgG) ELISA
Hepatitis A antibody (total or IgG) EIA
IgG: immunoglobulin G; ELISA: enzyme-linked immunosorbent assay; IU: international unit; IFA: immunofluorescence assay; EIA: enzyme immunoassay.
* Serologic testing for protective antibody to H. influenzae type b in international adoptees is not as well established as that for the other antibodies listed above.
¶ Value for positive result varies by test and laboratory.
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