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Example of fire ant rush protocol

Day	Volume (mL)	Dilution	Color vial
1	0.1	1:100,000
	0.3	1:100,000
	0.1	1:10,000	Silver
	0.3	1:10,000	Silver
	0.1	1:1000	Green
	0.3	1:1000	Green
	0.1	1:100	Blue
	0.3	1:100	Blue
2	0.1	1:10	Yellow
	0.2	1:10	Yellow
	0.3	1:10	Yellow
	0.4	1:10	Yellow
	0.5	1:10	Yellow
	0.1	1:1	Red
	0.2	1:1	Red
	0.3	1:1	Red

This protocol (Willford Hall protocol) was developed for administration of immunotherapy using whole body extract of imported fire ants.

Data from: Tankersley MS, Walker RL, Butler WK, et al. Safety and efficacy of an imported fire ant rush immunotherapy protocol with and without prophylactic treatment. J Allergy Clin Immunol 2002; 109:556.

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