Target cells (K562 cell line) are fluorescently labeled and incubated with increasing ratios of individual's peripheral blood mononuclear cells (PBMCs). Cytotoxicity is measured by staining of the labeled target cells with 7-AAD, an indicator of cell death, and cell size which decreases upon apoptosis.
HLH: hemophagocytic lymphohistiocytosis; %: percent; 7-AAD: 7-aminoactinomycin D.