Clostridium perfringens Gram stain

Gram stain of exudate from a bulla at the site of injection of intravenous drugs (x1000) shows large gram-positive bacilli and the absence of leukocytes. Clostridium perfringens grew anaerobically from this specimen on blood agar. Clostridial infection may produce cellulitis or involve deeper tissues, as in gas gangrene.