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Assessment of TLR4 pathway by flow cytometry

Normal individuals show induction of TNF-alpha in monocytes upon LPS stimulation. Individuals with IRAK-4 deficiency do not show optimal TNF-alpha expression.

TLR4: Toll-like receptor 4; IRAK-4: interleukin 1 receptor-associated kinase 4; TNF: tumor necrosis factor; LPS: lipopolysaccharide.

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