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Methacholine challenge dilution schedule

Methacholine challenge dilution schedule
Label strength Take Add NaCl (0.9%) Obtain dilution
Example of a dilution schedule for quadrupling concentrations
100 mg 100 mg 6.25 mL A: 16 mg/mL
3 mL of dilution A 9 mL B: 4 mg/mL
3 mL of dilution B 9 mL C: 1 mg/mL
3 mL of dilution C 9 mL D: 0.25 mg/mL
3 mL of dilution D 9 mL E: 0.0625 mg/mL
3 mL of dilution E 9 mL F: 0.015625 mg/mL
Example of a dilution schedule for doubling doses
100 mg 100 mg 6.25 mL A: 16 mg/mL
3 mL of dilution A 3 mL B: 8 mg/mL
3 mL of dilution B 3 mL C: 4 mg/mL
3 mL of dilution C 3 mL D: 2 mg/mL
3 mL of dilution D 3 mL E: 1 mg/mL
3 mL of dilution E 3 mL F: 0.5 mg/mL
3 mL of dilution F 3 mL G: 0.25 mg/mL
3 mL of dilution G 3 mL H: 0.125 mg/mL
3 mL of dilution H 3 mL I: 0.0625 mg/mL
3 mL of dilution I 3 mL J: 0.03125 mg/mL
Reproduced with permission from: Coates AL, Wanger J, Cockcroft DW, et al. ERS technical standard on bronchial challenge testing: general considerations and performance of methacholine challenge tests. European Respiratory Journal May 2017, 49 (5) 1601526; DOI: 10.1183/13993003.01526-2016. Copyright © 2017 European Respiratory Society.
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