Electrophoresis for hemoglobin H: Alkaline cellulose acetate gel

This is a hemoglobin electrophoresis run at alkaline pH on cellulose acetate gel. Lane 2 is a commercial standard containing approximately equal amounts of hemoglobins A, F, S, and C. Lane 1 is a hemoglobin sample from a patient with myelodysplastic syndrome/acute myeloid leukemia, a population of hypochromic-microcytic red cells, and acquired hemoglobin H disease. A minor fast-migrating band, hemoglobin H, is seen along with hemoglobin A.