- Place fresh stool on scrap paper or impervious tile.
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- Press mesh screen over stool to filter large particles.
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- Collect filtered stool using a small spatula and fill template (1.5 mm thick with hole 6 mm in diameter) resting on a glass slide.
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- Remove the template (leaving a 1.5 mm by 6 mm sample of stool).
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- Place a piece of cellophane (soaked in glycerol-malachite green or glycerol-methylene) over the stool sample as a cover slip.
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- Invert the slide and press down until the stool spreads evenly.
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- Place the slide in a rack for 30 minutes.
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- Count the number of eggs on the slide and multiply by 24 to determine total eggs per gram of stool.
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