Tests to establish the diagnosis | Additional tests/procedures at diagnosis |
Complete blood count and differential count | Demographics and medical history* |
Bone marrow aspirate | Detailed family history¶ |
Bone marrow trephine biopsyΔ | Patient bleeding history◊ |
Immunophenotyping | Performance status (ECOG/WHO score) |
Genetic analyses | Analysis of comorbidities |
Cytogenetics§ | Biochemistry, coagulation tests, urine analysis¥ |
Screening for gene mutations including:‡ | Serum pregnancy test† |
- FLT3, IDH1, IDH2, NPM1, CEBPA, RUNX1, FLT3, TP53, ASXL1
| Information on oocyte and sperm cryopreservation** |
Screening for gene rearrangements:¶¶ | Eligibility assessment for allogeneic HCT (including HLA typing)ΔΔ |
- PML-RARA, CBFB-MYH11, RUNX1-RUNX1T1, BCR-ABL1, other fusion genes (if available)
| Hepatitis A, B, C; HIV-1 testing |
Sensitive assessment of response by RT-qPCR or MFC◊◊ | Chest radiograph, 12-lead electrocardiogram, and echocardiography or MUGA (on indication) |
RT-qPCR§§,¥¥ for NPM1 mutation, CBFB-MYH11, RUNX1-RUNX1T1, BCR-ABL1, other fusion genes (if available)◊◊ | Lumbar puncture‡‡ |
Multiparameter flow cytometry (MFC)¥¥,†† | Biobanking*** |